Antimicrobial dressing efficacy against mature Pseudomonas aeruginosa biofilm on porcine skin explants

Methods: The assessments were performed on biofilm attached to dermal tissue using an ex vivo porcine skin explant biofilm model. Its use as a dermal substrate for attachment and the primary source of nutrition provides an alternative cost-effective method that more closely mimics in vivo conditions, with the advantages of an in vitro system, to assess the antimicrobial efficacy of treatments against mature biofilms. This ex vivo porcine skin explant biofilm model has been further validated against data generated from an in vivo pig burn wound model that was used to assess the efficacy of silver dressings against PAO1 biofilms. Five types of antimicrobial agents and four types of moisture dressings were assessed.